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Comparative Study of the L-Hydro Process and Glutaraldehyde Preservation

The Heart Institute (HC-InCor), University of São Paulo Medical School, São Paulo, Brazil
¹ The International Heart Institute of Montana, Missoula, MT, USA

For reprint information contact: Vinicius JS Nina, PhD Tel: 55 98 3227 9191 Fax: 55 98 3232 8700 Email: rvnina{at}aol.com, Rua Sebastião Archer, 101 Olho D’ Água, CEP: 65065-480, São Luís-MA, Brazil.

Commercial bioprosthetic heart valves are commonly preserved in glutaraldehyde and are cytotoxic to host cells, preventing spontaneous endothelialization. The aim of this study was to demonstrate the potential for in vivo endothelialization of bioprostheses treated by the L-Hydro process which consists of mild extraction of antigenic substances and incorporation of antiinflammatory and antithrombotic agents. Seven stented porcine heart valves treated by the L-Hydro process and 3 glutaraldehyde-fixed porcine heart valves were implanted in the mitral position in juvenile sheep. The valves were evaluated by echocardiography, angiography, histology, and histochemistry. No hemodynamic differences were observed, but scanning and transmission electron microscopy showed nearly complete coverage by endothelial cells of all leaflets in the L-Hydro-treated valves after 5 months of implantation. The endothelial cells were in direct contact with the underlying collagen and expressed von Willebrand-related antigens. The surfaces of the glutaraldehyde-treated valves were covered by fibrin, macrophages, calcium, and thrombotic material; only sparse endothelial cells were observed and contact with the underlying tissue was incomplete. These data indicate that L-Hydro-treated porcine valves are capable of inducing spontaneous endothelialization.